INVESTIGATION OF LIPOPHILIC COMPONENTS OF RHEUM TATARICUM L.f.
DOI:
https://doi.org/10.51580/2025-3.2710-1185.33%20Keywords:
Rheum tataricum L.f., lipophilic compounds, gas chromatography-mass spectrometry (GC-MS), β-sitosterol, steroids, terpenes, hexane extract, extraction, aerial part, underground partAbstract
Despite the widespread use of Rheum tataricum L.f. (genus Rheum L., family
Polygonaceae Juss.) in phytotherapy, its lipophilic components remain insufficiently studied. In this
study, we analyzed the lipophilic composition of hexane extracts obtained from Rheum tataricum L.f.
Plant material was harvested during the fruiting phase in the Almaty region. Metabolites were determined
by gas chromatography-mass spectrometry (GC-MS). The Agilent MSD ChemStation software (version
1701EA) was used to control the gas chromatography system, record and process the obtained results and
data. As a result of the study, 11 lipophilic components were found in the underground part, of which 7
were identified; in the aboveground part – 23 components, 12 compounds were identified. The main
metabolites of the hexane extract obtained from the underground part of the plant are β-sitosterol
(63.23%), campesterol (11.94%) and octacosanol (8.45%). Steroid components predominate among the
identified compounds. In the aboveground part, β-sitosterol (28.92%), squalene (16.22%), γ-tocopherol
(11.73%) and α-amyrin (4.64%) are dominant. The total proportion of identified components contained in
the hexane extracts for the underground part was 91.20%, and for the aboveground part – 76.29%,
respectively. Terpenoid compounds predominate in the studied extracts, which indicates potential
biological activity and value for conducting further research.
