ANALYSIS OF PHENOLIC COMPOUNDS AND BIOLOGICAL ACTIVITY OF PLANTS OF THE GENUS CLIMACOPTERA BRACHIATA

Abstract

Abstract. Climacoptera brachiata is an annual species of halophytes with great potential for use in
urban landscapes. Extracts of Climacoptera brachiata have been used in traditional medicine for many
years due to their antifungal, antibacterial activity due to various biologically active compounds. It is
known that the leaves contain triterpenoids, and the flowers contain phenolic compounds.The authors
proposed a scheme for the analysis of water-alcohol extracts of Climacoptera brachiata. In addition,
extracts were screened and determined by high-performance liquid chromatography. For the simultaneous
analysis of phenolic compounds, a fast and sensitive method for the determination of natural compounds
using high-performance reverse phase liquid chromatography (RP-HPLC) in combination with a diode
matrix detector (DAD) has been developed. Gradient elution systems of 0.1% acetic acid and methanol
were linearly used, the analysis time was 57 minutes. The method was confirmed by linearity, relative
error, reproducibility, and LOD values. The detection of compounds was carried out at 254 nm. The
developed method for the rapid determination of phenolic compounds using RP-HPLC was used to
determine the presence of phenolic compounds in natural and commercial products.