ISOLATION OF GALLIC ACID FROM LINOSYRIS VILLOSA PLANT, SYNTHESIS OF ITS DERIVATIVES AND STUDYING THEM FOR ANTIMICROBIAL ACTIVITY

Abstract

The research focuses on finding cheap, eco-friendly antibacterial agents based on local plant materials. Gallic acid and its derivatives were selected as objects of biological active substances of plant raw materials. The aim of this work was to isolate Gallic acid from the Linosyris villosa plant growing in the north of Kazakhstan and to obtain dibromogallic acid and dinitrogallic acid on its basis. The structure of the obtained compounds was proved using thin-layer chromatography, measuring the melting point and the method of IR spectroscopy. Gallic acid and its derivatives, dinitrogallic and dibromogallic acid, were studied for antimicrobial activity against Escherichia coli, Staphylococcus aureus, dysentery bacillus, Salmonella. The study of Gallic acid and its derivatives: Dibromogallic and Dinitrogallic acids for antimicrobial activity showed that Gallic acid does not exhibit antimicrobial effect, while dinitrogallic acid has an antimicrobial effect only on solid media (meat-peptone agar) and is inactive in a liquid nutrient medium. The best indicators of antibacterial activity were shown by Dibromagallic acid solutions: a complete cessation of the pathogenic bacteria colonies growth on solid media and lysis of the studied cultures of microorganisms in a liquid nutrient medium were observed.

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