METHODS OF PRODUCTION OF AMINO ACIDS AND PROTEINS, LABELED WITHSTABLE ISOTOPЕS 2N, 13C, 14C, 15N, 18O

Abstract

The tendencies towards the preferred use of stable isotopes in comparison with their radioactive counterparts are due to the absence of radiation hazard and the possibility of determining the localization of the label in the molecule by high-resolution methods. That is why the development of ways to obtain amino acids and proteins labeled with stable isotopes is an urgent task for modern biotechnology. These isotopically labeled biologically active compounds (BACs) obtained by this method with different levels of isotopic enrichment, from selectively to uniformly labeled, are convenient tools for various metabolic and biochemical studies of medical diagnostics of various diseases of chemical syntheses of various isotopically labeled compounds based on them.This review covers modern biotechnological and enzymatic methods for the synthesis of amino acids and proteins, stable isotopes 2H, 13C, 15N, 18O. New perspectives for the application of described methods of amino acid synthesis and proteins, stable stable isotopes were considered. In particular, there are examples of the use of methods of nuclear magnetic resonance, infrared and laser spectroscopy, as well as mass spectroscopy in the process of biochemical synthesis of the above-mentioned compounds and the further information provided.

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