STUDY OF ANTIOXIDATIVE ACTIVITY OF EXTRACTS FROM WASTES OF CARBON DIOXIDE EXTRACTION OF SCABIOSA OCHROLEUCA L. BY WAY OF INVERSE VOLTAMMETRY

Abstract

We have studied the antioxidative activity (АОА) of water and alcoholic extracts from waste of carbon dioxide extraction of top part of cream scabious (Scabiosa ochroleuca L.). Study of АОА of water and alcoholic extract from waste of carbon dioxide extraction of herb of cream scabious was made by way of inverse voltammetry. Using the method of cathode voltammetry to carry out one experiment, the sample of extract of 0,3 g was taken. The direct current mode of cathode voltammetry was applied, scanning velocity W = 40 mV/s, operational range of potentials 0,0 to -1 V. Electrochemical cell was represented by glass bowl with solution of bulk electrolyte with immersed indicative mercury-film electrode, chloride-silver reference electrode and chloride-silver electrode. As the base solution – phosphate buffer with рН 6,6. Ratio of change of current of EV О2 was the indicator of activity of sample under study. Water and alcoholic extracts from waste of carbon dioxide extraction (СО2-residues) was obtained by infusion in ethyl alcohol of 70% and distilled water; for this 350 g of air-dry weight of СО2-resideus of cream scabious was taken and put to the container of stainless steel. Ethyl alcohol of 70% / distilled water was added, and infused for 3 days. After this, it was filtered to round-bottom flask with slice (250 ml) and distilled the solvent with rotary evaporator. Extracts concentration from waste of carbon dioxide extraction was carried out with rotary evaporator IKARV 10 Digital with temperature range of 40-45 0С, on the base of Scientific and research institute «Novye materialy» of Karaganda State Technical University.

References

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