DEVELOPMENT OF A BIOACTIVE COMPLEX WITH ANTIINFLAMMATORY PROPERTIES

Abstract

The article presents the results of phytochemical studies conducted on tomato fruits
(Solanum lycopersicum L.) and garlic cloves (Allium sativum L.) collected near Almaty (Kazakhstan), as
well as jack bean seeds (Canavalia ensiformis (L.) DC.) and turmeric rhizomes (Curcuma longa L.)
collected near Karachi (Pakistan). The study of biologically active compounds revealed a high level of
phenols (2.02–2.86%) across all plant samples. Additionally, 3.34% of total amino acids were found in the
seeds of C. ensiformis, while C. longa rhizomes contained 1.15% tannins. Colorimetric, chromatographic,
and spectrophotometric methods were employed to determin0e chemical composition. In order to create a
biologically active complex, four different ratios of plant raw materials were studied as a result of which a
high content of extractive substances of the ASCC-2 composition (44.65%) was found in a 50% aqueous
alcohol solution. The effective technological parameters that can be used to obtain a tank are considered.
The ratio of raw materials and solvent 1:10, 40 °C, 60 min was determined by the optimal parameters
during ultrasonic extraction of the ASCC-2 composition. The resulting extract was concentrated in a
rotary evaporator at 40 °C, and then sent to a freeze dryer. The dry extract is colorless, well soluble in
water, the pH value is 5.9. BAC showed high anti-inflammatory activity (IC₅₀ = 5.4±1.2 µg/ml).