Chemical Journal of Kazakhstan

REVIEW OF ELECTROCHEMICAL BIOSENSORS BASED ON CARBON NANOMATERIALS FOR EARLY CANCER DIAGNOSIS

2026-03-11T15:38:51+05:00

Abstract. Cancer is one of the leading causes of mortality worldwide. Early diagnosis of tumors is considered a key and at the same time challenging task in the effective treatment of oncology patients. In recent years, nanomaterial-based biosensors have been rapidly developing as modern and highly sensitive tools for cancer diagnostics. In particular, carbon nanomaterials significantly enhance the analytical performance of electrochemical and optical sensing systems.
The aim of this review is to systematize the capabilities of electrochemical and optical biosensors based on carbon nanomaterials for early cancer detection. The main objectives include analyzing the properties of the applied carbon nanostructures (graphene, graphene oxide, carbon nanotubes, and carbon quantum dots) and evaluating their efficiency in the determination of tumor markers. A comprehensive analysis of the scientific literature was conducted, and methods such as voltammetry, amperometry, electrochemical impedance spectroscopy, electrochemiluminescence, and surface plasmon resonance were considered. Special attention is paid to strategies for bioreceptor immobilization and nanocomposite functionalization. Carbon nanomaterials exhibit high electrical conductivity, large specific surface area, and good biocompatibility. These properties enable the detection of tumor markers (CEA, AFP, miRNA, and proteins) at low concentrations. The sensors are characterized by portability, rapid response, and low limits of detection. Biosensors based on carbon nanomaterials have high practical significance in the field of early diagnosis and demonstrate strong potential for clinical application.

STABILIZATION OF IRON NANOPARTICLES BY BIOPOLYMERS DURING CHEMICAL REDUCTION WITH SODIUM BOROHYDRIDE

2026-06-15T12:33:45+05:00

Abstract. Introduction. One of the effective ways to regulate the processes of formation and agglomeration of FeNPs is the use of biopolymer stabilizers. The goal is to investigate the influence of biopolymer stabilization on the size, morphological and crystalline characteristics of FeNPs obtained by chemical reduction of NaBH4. Methods. FeNPs were synthesized by reduction of Fe3+ with NaBH4, stabilized with chitosan and sodium carboxymethylcellulose. The optical properties were studied using UV-Visible spectrophotometry, FTIR to identify functional groups, SEM-EDS and TEM to examine morphology and elemental composition, XRD to determine crystalline phases, and DLS-ELS to evaluate hydrodynamic diameter and ζ-potential. Results and discussion. UV-Vis spectra showed a characteristic 260-300 nm absorption band of iron-containing nanostructures. FTIR spectra indicated Fe-O bonds and interactions between FeNPs and biopolymer functional groups. SEM analysis showed reduced aggregation in FeNPs/Na-CMC and FeNPs/CS compared with unstabilized FeNPs. EDS analysis confirmed the presence of Fe and O elements and the formation of an oxide/hydroxide phase. XRD analysis revealed the formation of crystalline iron oxide phases. The average-sized crystallites are approximately 18 nm for FeNPs, 14 nm for FeNPs/Na-CMC, and 10 nm for FeNPs/CS. TEM and DLS/ELS confirmed the formation of spherical FeNP/CS nanoparticles with a size range of 20.03 ± 3.62 nm and a zeta-potential of -30.6 mV.Conclusion. Biopolymer stabilizers were found to reduce the growth and aggregation of FeNPs. CS effectively limits crystallite growth and suppresses agglomeration, while Na-CMC forms structurally organized composite particles. The results demonstrate the potential of using biopolymers to stabilize iron-containing nanomaterials.

REGENERATION AND CATALYTIC PERFORMANCE OF NI-MO-AL-HMS-H-BENTONITE CATALYST IN HYDROGENATION OF AROMATIC HYDROCARBONS

2026-06-15T09:16:52+05:00

Abstract. Introduction. The physico-chemical and catalytic properties of the bifunctional catalyst Ni-Mo-Al-HMS-H-bentonite were investigated in the hydrogenation of a model mixture of 2-methylnaphthalene and dibenzothiophene. This study aimed to compare the properties of fresh and regenerated catalysts and evaluate their efficiency in this reaction. Results and discussion. Catalyst physico-chemical parameters were analyzed by X-ray photoelectron spectroscopy, temperature-programmed hydrogen reduction, nitrogen adsorption-desorption analysis and thermogravimetric analysis. It was established that nickel and molybdenum are mainly present on the catalyst surface in oxide and sulfide forms that determine its catalytic activity. Textural analysis showed that the catalyst possesses a well-developed mesoporous structure with a monomodal distribution of pore sizes. After the catalytic reaction and regeneration, a decline in the textural characteristics were evaluated. Catalytic tests were carried out at 260°C, hydrogen pressure of 6 MPa and reaction time of 5 hours. The fresh catalyst maintained stable activity for four reaction cycles, while the regenerated catalyst demonstrated high efficiency for three subsequent cycles. Conclusion. The obtained results confirm the potential application of the Ni-Mo-Al-HMS-H-bentonite catalyst in hydrogenation processes of aromatic and sulfur-containing compounds.

INFLUENCE OF CEFAZOLIN LOADING ON THE STRUCTURE AND INTERFACIAL ACTIVITY OF CHITOSAN-BASED NANOGELS

2026-06-01T11:05:35+05:00

Abstract: Chitosan-based nanogels are promising colloidal carriers for hydrophilic drugs de too their biocompatibility, adjustable physicochemical properties, and mild preparation conditions. In this study, cefazolin-loaded chitosan nanogels were prepared by ionic gelation using sodium tripolyphosphate (TPP) as a crosslinker. The effect of drug incorporation on the colloidal characteristics and interfacial behavior of the system was systematically evaluated. Freshly prepared blank and cefazolin-loaded nanogels showed hydrodynamic diameters of about 210 nm and 285 nm, respectively. Both systems exhibited positive zeta potential values above +25mV, indicating good electrostatic stability under acidic conditions. The encapsulation efficiency of cefazolin, determined by an indirect UV-Vis method, was 48 ± 6%, confirming effective drug entrapment within the crosslinked polymer network. After dialysis to remove low-weight impurities, purified dispersions were analyzed by dynamic surface tension measurements using the pendant drop method. Blank nanogels gradually adsorbed at the air-water interface, reducing surface tension from 71.8 to 67.4 mN/m ver time. In contrast, cefazoli-loaded nanogels exhibited slower adsorption kinetics and a smaller decrease in surface tension, reahing equilibrium values around 68.9mN/m.These differences are attributed to increased hydration and reduced mobility of chitosan chains after drug incorporation. Overall, cefazolin encapsulation significantly modified the interfacial adsorption behavior of chitosan nanogels while maintaining their colloidal stability. The results provide insight into structure-property relationships relevant to aqueous and topical drug delivery applications.

STUDY OF THE SORPTION OF NI²⁺, CO²⁺, AND V⁴⁺ CATIONS IN A THREE-COMPONENT SYSTEM USING MODIFIED ZEOLITE

2026-03-30T11:26:16+05:00

Introduction. Cobalt, nickel, and vanadium are toxic heavy metals that can concentrate and accumulate in soil, wastewater, groundwater, and even in the human body. One of the effective ways to address these problems is the use of accessible adsorbents capable of purifying various types of water. According to the analysis of literature data, the most suitable sorbents are natural and modified forms of zeolites. The aim of the study was to investigate the sorption properties of nickel(II), cobalt(II), and vanadium(IV) ions using the saturation method from aqueous solutions with zeolites modified by guar gum. Results and discussion. The sorption efficiency of the modified zeolite in multimetal systems was investigated, and it was established that the obtained sorbent simultaneously absorbs all cations. It was shown that, unlike natural zeolite, a high degree of sorption by the modified zeolite is achieved both for a cation with a fixed concentration and for one with a variable concentration. The degree of uptake of the sorbed cations is determined by the combination of two cations with constant and equal contents and the concentration of the variable cation. In all the studied systems, a high degree of cation sorption (from 75.0 to 99.5%, depending on the process conditions) occurs in the region with a low concentration of the variable cation (0.5–10 mg/L). Conclusion. It was revealed that in multimetal systems competitive adsorption of cations occurs. Series of sorption efficiency for the cations were obtained, as well as data on the initiating influence of the variable cation—under conditions of its increased concentration—on the sorption of other ions present in the system. In the system “Ni²⁺ – Co²⁺ – V⁴⁺ – MPC – H₂O” with variable C(Co²⁺) and saturated solutions, the Co²⁺ cation initiates the sorption efficiency of the modified sorbent toward V⁴⁺ and Ni²⁺ cations: RV (66.6%) > RCo (42.4%) > RNi (13.4%). With variable C(Ni²⁺), the Ni²⁺ cation increases the sorption efficiency of V⁴⁺ and Co²⁺ ions: RV (63.6%) > RCo (1.8%) > RNi (3.8%).

STUDY OF THE SORPTION CHARACTERISTICS OF MODIFIED ZEOLITE

2026-03-27T16:48:46+05:00

Introduction. Contaminated industrial wastewater contains carcinogenic metals that negatively affect human health. Research in the field of purification of liquid media polluted with heavy metals focuses on the use of efficient, accessible, inexpensive, and environmentally friendly sorbents and sorption materials based on natural raw materials, in particular zeolite. Natural zeolites from different deposits vary in their mineralogical composition and are characterized by less effective sorption properties compared to synthetic sorbents. To improve the sorption characteristics, an inexpensive modifier guar gum has been selected. The aim of the work. Modification of natural zeolite with guar gum by the precipitation method and determination of the optimal conditions under which the highest sorption characteristics are achieved. Results and discussion. Study of the effect of guar gum consumption on the properties of modified zeolite showed that with an increase in the amount of modifier, the static exchange capacity (SEC) of the cation exchanger sharply decreases. The optimal stirring time for obtaining the modified sorbent was found to be 10 hours, at which the maximum SEC value of the cation exchanger reaches 1398 mg-eq/g. Investigation of the effect of stirring temperature showed that in the range from 25°C to 75°C, the sorption characteristics of the composite sorbent change insignificantly. An increase in the drying temperature to 100°C leads to an improvement in the sorption characteristics of the sorbent, especially with respect to the sorption of cations. Conclusion. The conditions for modifying natural zeolite with a natural polysaccharide (guar gum) by the precipitation method have been studied. Optimal conditions for the modification process have been determined, and a new sorbent has been obtained in which the cation exchange capacity, anion exchange capacity, overall exchange capacity, and total sorption capacity are 15 times, 85.6 times, 9.7 times, and 77 times higher, respectively, compared to natural zeolite.

CATALYTIC PROCESSING OF NATURAL BITUMEN FROM BITUMINOUS SANDS

2026-05-14T12:12:32+05:00

Introduction. Bituminous sands deposits are a very rich source of energy in bitumen. However, access to the main part of these reserves is difficult, and the conversion of bitumen into liquid, low-sulfur products require innovative solutions, since the properties of traditional oil and bitumen are significantly different. Methods. To obtain the necessary products from bituminous sands, it is first necessary to separate organic part from the bituminous sands, and only then it can be further processed. Currently, industrial bitumen production in Canada is carried out using mining technology. In this regard, Kazakhstan needs to develop domestic technology in accordance with its West Kazakhstan conditions and domestic demand. Results and discussion. High-temperature cracking processes of West Kazakhstan natural bitumens in the presence of mesoporous aluminosilicate, synthetic zeolite and natural zeolite catalysts have been studied. The catalysts lead to the formation of liquid products up to 92.2%, reducing the number of tar-asphaltene compounds from 52.4% to 16.3%. Conclusion. Natural bitumen from the Beke and Munaily Mola fields is classified as heavy oil and consists mainly n-alkanes, terpanes, and steranes. A mesoporous aluminosilicate catalyst enabled efficient catalytic cracking, giving up to 89% liquid products and increasing oil and light fraction yields. High activity is attributed to the large pore size. Cracking destroys aliphatic and polynaphthenic structures in asphaltenes, increases aromaticity, and reduces oxygen-containing compounds, while resins show increased aromaticity due to the breakdown of naphthenic rings and alkyl groups.

HYDROGENATION OF TOLUENE TO METHYLCYCLOHEXANE OVER PROMOTED SKELETAL NICKEL CATALYSTS

2026-06-17T11:22:42+05:00

Introduction. Methylcyclohexane (MCH) is a key solvent and a promising Liquid Organic Hydrogen Carrier (LOHC). The selective hydrogenation of toluene to MCH requires efficient, stable and cost-effective catalysts. Skeletal nickel (Raney Ni) is widely used but suffers from rapid deactivation. The purpose of this work is to develop multicomponent skeletal nickel catalysts modified by industrial ferroalloys (FeMo, FeTiMn, FeMn) and to study their catalytic performance in toluene hydrogenation. Methods. Catalysts were prepared by high-frequency induction melting of Ni-Al-ferroalloy systems, followed by leaching with 20% NaOH. Surface morphology and composition were characterized by SEM-EDXRS and BET. Kinetic experiments were carried out in a high-pressure autoclave (0.25 L) at 393–473 K and hydrogen pressures of 2.0–12.0 MPa. Results. The addition of 3.0 wt.% FeMo or 5.0 wt.% FeTiMn increased the hydrogenation rate by 2.2–2.5 times compared to unmodified Raney Ni. Specific surface area reached 82.1 m²/g for Ni-Al-FeMo and 78.5 m²/g for Ni-Al-FeTiMn. SEM-EDXRS showed uniform distribution of Fe, Mo, Ti, Mn in the nickel matrix. The reaction order was zero with respect to toluene and first with respect to H₂ (at 2-6 MPa). Apparent activation energy was 34.5 kJ/mol for Ni-Al-FeMo and 38.2 kJ/mol for Ni-Al-FeTiMn. The catalysts exhibited 99.9% selectivity to MCH and maintained >92% of initial activity after 100 h on stream. Conclusion. Ferroalloy-modified skeletal nickel catalysts are highly efficient, selective and stable, making them promising for industrial hydrogen storage and petrochemical applications.

SYNTHESIS OF IONITE BASED ON EPOXY RESINS AND POLYAMINES USING NEW INITIATING SYSTEMS FOR METAL SORPTION

2026-05-25T10:45:36+05:00

Abstract. Purification of drinking water, isolation of rare metals from industrial wastewater, and water treatment for a wide variety of applications are constant tasks requiring various solutions. Population growth, the development of new territories, agriculture, factories and manufacturing enterprises, all of them need clean water. Ionite is an ion–exchange resin, one of the ways to solve the described problems. Ionite is able to sort various ions from solutions, thereby purifying them or releasing valuable ions during sorption. The urgent task is to produce ionites more economically with minimal energy and material costs. The process of ionite synthesis consists in polymerization and copolymerization of various monomers, while introducing various initiators and changing the process conditions, it is possible to achieve a product with high characteristics.
The ionite was based on well-known monomers such as epoxy resins and aliphatic polyamines. The reagents used were (AGE) allylglycidyl ether, (ED-20) epoxy resin, and (PEI) polyethyleneimine. A product was synthesized from diglycidyl ether of dioxydiphenylpropane, allylglycidyl ether, and polyethylenimine using initiating systems based on potassium persulfate (K2S2O8) and azobisisobutyronitrile (AIBN). The synthesis process and method of introducing the initiator into the reaction mass were selected. The reaction was carried out in an organic solvent medium (DMF) - dimethylformamide. These initiators have not been described in known sources in combination with specific reagents during the synthesis of the ion exchanger. Further, the properties of a weakly basic anion exchanger were investigated using various methods, gravimetric, acid-base titration, infrared spectroscopy, atomic emission spectral analysis. The study of sorption capacity was carried out on the ions of metavanadate (VO3-). According to the content of vanadium - (V), in the solution before and after sorption, the sorption capacity of the ionite - (CE) in mg/g was calculated.
The resulting ionite can solve the problems of water purification and industrial wastewater treatment. It can be used for the selective extraction of non-ferrous and rare metals contained in industrial wastewater.

SOFT ORGANOMODIFICATION OF BENTONITE WITH OXYPHOSPHONATE AND ITS APPLICATION AS A PROLONGED-LASTING GROWTH STIMULATOR

2026-05-05T16:19:35+05:00

Introduction. Organomodified clay materials are of significant interest as functional systems for agriculture due to their ability to provide the sustained release of biologically active substances. Natural bentonite, with its high specific surface area and ion-exchange capacity, serves as an excellent matrix for the immobilization of organic growth stimulants. The purpose of this study is to develop a low-energy, efficient method for the soft organomodification of bentonite using a specific oxyphosphonate (Kaz-6) and to evaluate its effectiveness as a prolonged-action growth stimulator for wheat. The proposed method involves the pre-dispersion of bentonite in water at 40°C, followed by drying at 50°C. The prepared mineral matrix was then treated with low-concentration aqueous solutions of dimethyl(4-hydroxy-1-(2-ethoxyethyl)piperidin-4-yl)phosphonate (Kaz-6) in the concentration range of 10^-2–10^-6 wt % at room temperature. The structure of the resulting composite was characterized using IR spectroscopy. It was found that the developed approach significantly reduces the process temperature and duration compared to existing analogs, while using substantially lower concentrations of the organic component. Biological trials showed that the organomodified bentonite exhibits pronounced growth-promoting activity toward wheat (varieties “Almaken” and “Zhenis”). The most significant stimulatory effect was observed at concentrations of 10^-5–10^-6 wt. %, where the composite outperformed both the control group and the free (unbound) oxyphosphonate. The results indicate that the immobilization of Kaz-6 on bentonite ensures prolonged action and increased bioavailability of the active component. The developed organomineral composite can be recommended as a promising growth-promoting agent for sustainable agricultural applications.

PHYTOTOXICITY OF THE BIOLOGICALLY ACTIVE COMPOUNDS LIBRARY CONSISTING OF O-para-TOLUOYL-β-(MORPHOLIN-1-YL)PROPIOMIDOXIME SALTS AND 5-ARYL-3-β-(PIPERIDIN-1-YL)ETHYL-1,2,4-OXADIAZOLES

2026-06-18T09:42:12+05:00

Abstract. Introduction. Agrochemical use increases environmental and health risks, making phytotoxicity studies essential. Amidoximes and 1,2,4-oxadiazoles are exogenous sources of NO, which can reduce abiotic stress and the phytotoxicity of agrochemicals; nevertheless, their phytotoxic profile has been poorly studied. Goals and objectives. To assess phytotoxicity of water-soluble compounds: O-para-toluoyl-β-(morpholin-1-yl)propioamidoxime salts (hydrochloride, oxalate, citrate) and three 5-aryl-3-β-(piperidin-1-yl)ethyl-1,2,4-oxadiazoles (aryl: para-MeC₆H₄, para-BrC₆H₄, meta-ClC₆H₄). Methods. Compounds were synthesized via improved multi-step synthesis. Phytotoxicity was tested on Lactuca sativa L. root and stem lengths at 100, 500, 1000 μg/ml. Results. Phytotoxicity depended on concentration and organ; roots were more sensitive. The highest phytotoxic effect was observed for para-bromo-oxadiazole with phytotoxicity from 57-67% at 500-1000 μg/ml 33-72%, followed by meta-chloro-oxadiazole with phytotoxicity from 33-72% at 100-1000 μg/ml. A growth-stimulating effect of amidoxime chloride and oxalate on stem growth is noted. Moderate toxicity was seen for hydrochloride salt and para-methyl oxadiazole. Citrate salt exhibited minimal phytotoxicity, comparable to control. Conclusion. The studied compounds showed different degrees of phytotoxicity depending on concentration and plant organ; roots were more sensitive with increasing concentration. Phytotoxicity is maximum for oxadiazoles with halogen substituents (para-bromo, meta-chloro), moderate/minimal for para-methyl oxadiazole and amidoxime hydrochloride and citrate. Shoot growth was stimulated by amidoxime hydrochloride and oxalate.

BENZOYLATED BISPIDONE: SYNTHESIS AND LOCAL ANAESTHETIC ACTIVITY

2026-06-17T10:00:07+05:00

Introduction. Local anaesthetics work by blocking voltage-gated sodium channels, which causes a temporary loss of sensation needed for many medical procedures. While novocaine, lidocaine, and trimecaine are effective, they have drawbacks such as short duration, possible toxicity, and tolerability problems. This study set out to create a complex of benzoylated 3-(3-butoxypropyl)-7-cyclopropanemethyl-substituted bispidone with β-cyclodextrin (LAC-5) and to compare its local anaesthetic effects with medicals using standard preclinical tests. LAC-5 was synthesised using Mannich condensation, oximation, O-benzoylation, and β-cyclodextrin complexation. Its structure was confirmed by methods such as infrared and nuclear magnetic resonance spectroscopies. Tests showed that LAC-5 has strong local anaesthetic effects, performing better than trimecaine, lidocaine, and novocaine in both infiltration and conduction anaesthesia models. In infiltration anaesthesia, LAC-5 achieved the maximum measurable depth of anaesthesia (index 36.0) at a 0.25% concentration, with a duration of complete anaesthesia of 56.66 min and a total duration of action of 76.66 min, exceeding the reference medications by up to 5.66- and 2.6-fold, respectively (p < 0.001). In conduction anaesthesia, LAC-5 produced a total duration of action of 160.0 ± 4.7 min, surpassing reference medications. Based on the results obtained, LAC-5 is recommended for comprehensive preclinical pharmacological evaluation, including assessment of acute toxicity, safety profile, and mechanism of action.

DETERMINATION OF THE COMPONENT COMPOSITION OF A SERIES OF CALCIUM-CONTAINING DEPOSITS AND TECHNOLOGICAL RECOMMENDATIONS FOR THEIR UTILIZATION

2026-05-21T14:52:12+05:00

Abstract. A comprehensive analysis of samples of calcium-containing mineral raw materials from deposits in Russia (Bryansk), Kazakhstan (Beineu, Shetpe, Zhanakorgan), and Uzbekistan (Fergana) was carried out to assess their suitability as precursors of calcium oxide used in technologies for the remediation of oil-contaminated soils. The study employed methods of X-ray phase analysis (XRD), elemental analysis, and infrared spectroscopy (FTIR). By the XRD method, the phase composition of the samples was established and their semi-quantitative evaluation was performed. Based on the obtained data, the samples were classified into three groups: 1) calcium oxide–hydroxide (Bryansk, Beineu, Fergana), characterized by a high content of CaO (76.1-92.5%) and Ca(OH)₂ (7.5–19.0%); 2) calcium hydroxide (Zhanakorgan), representing almost pure Ca(OH)₂ (99.6%); 3) carbonate (Shetpe), consisting of calcite CaCO₃ (>98%). FTIR results confirmed the presence of the identified phases. Elemental analysis showed high purity of most samples in terms of calcium (>52% CaO in normalized form). The sample from Bryansk contains an increased amount of silica (SiO₂ ≈ 4%). Technological recommendations for the use of the raw materials are proposed. The Beineu-1 and Beineu-2 samples, containing 92.5% and 81.0% CaO, respectively, were identified as the most promising raw materials for the energy-efficient production of active CaO.

SYNTHESIS AND PHYSICOCHEMICAL CHARACTERIZATION OF CHITOSAN–SiO2-BASED COMPOSITES

2026-05-21T08:38:55+05:00

Introduction. Composites based on chitosan are of particular interest owing to their biodegradability, environmental safety, and strong affinity for metal ions. Immobilization of chitosan on an inorganic support such as silica can be used as a method to improve physicochemical properties of hybrid materials such as their thermal stability and mechanical strength. Objectives. The main purpose of this work was preparation of chitosan–SiO₂ composites with different chitosan loadings and determination of their physicochemical properties. Methods. Composite chitosan–SiO₂ with 5, 10, and 20 wt.% content of chitosan was synthesized via adsorption immobilization followed by alkaline precipitation. Physical characteristics of obtained materials were studied using methods such as thermogravimetric analysis (TGA), nitrogen adsorption–desorption analysis (BET), and scanning electron microscopy (SEM). Results and discussion. In our study, we showed that alkaline precipitation greatly increased the efficiency of adsorption and immobilization of chitosan, giving immobilization yields of 93-100%. From thermogravimetric analysis, it was found that content of organic substances in the materials is gradually increased by increasing chitosan loading. According to the classification of the IUPAC, the isotherm is classified as type IV isotherms with H3 hysteresis loop characteristic for mesoporous materials. Scanning electron microscopy revealed heterogeneous and relatively rough surface morphology. Conclusion. Obtained composite materials have developed mesoporous structure, high thermal stability, and good immobilization of chitosan, which allows to consider them as prospective sorbents for heavy metal ion elimination from aqueous solutions.

VALIDATION OF THE METHOD FOR DETERMINING THE CONCENTRATION OF IODIDE IONS IN DIFFERENT DOSAGE FORMS BY UV SPECTROPHOTOMETRY

2026-06-15T15:09:04+05:00

Abstract. This article is devoted to the validation of an analytical method for the determination of iodide ions in various dosage forms using UV spectrophotometry. This method is an important part of analytical chemistry and drug quality control systems, in which the sensitivity, specificity, and reproducibility of the method are crucial. The relevance of the study is determined by the need for accurate and reliable quantitative determination of iodide ions in pharmaceutical substances and finished drug products. Validation was conducted in accordance with the requirements of the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH Q2(R1)/Q2(R2)). The aim of this study was to determine the validation characteristics of a method for the quantitative determination of iodide ions in dosage forms using UV spectrophotometry. Results and discussion. The following parameters were evaluated: specificity, linearity, working range, accuracy, repeatability, within-laboratory precision, and solution stability. The working range was found to be from 20 to 240 mg/L, and the correlation coefficient was 0.9998. The method demonstrated high sensitivity and compliance with international requirements. Conclusion. The results obtained confirm the linearity of the method across the studied range of iodide ion concentrations. High sensitivity and a high degree of linearity (R² = 0.9998) were demonstrated. The developed method is suitable for its intended purpose and can be successfully applied in the analysis of iodine-containing medicinal products. The method has practical significance for pharmaceutical quality control systems.